pENTR-L5-oPRE-L2
(Plasmid #32414)

• Depositing Lab: Matthew Nolan
• Publication: White et al Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4.

Backbone

• Vector backbone: pDONR221 P5-P2
• Backbone manufacturer: Invitrogen
• Vector type: Gateway cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: oPRE
• Alt name: optimised version of WPRE
• Mutation: WPRE with deletion of X protein promoter and mutation of all four ATGs which are followed by an ORF encoding more than 25 amino acids

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pMP71 GFP oPRE Provided by Dr Axel Schambach the user of the oPRE should cite the original article describing the element by Axel Schambach of Hannover Biomedical Research School
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gene Therapy (2006) 13, 641–645. doi:10.1038/sj.gt.3302698; published online 15 December 2005

'Woodchuck hepatitis virus post-transcriptional regulatory element deleted from X protein and promoter sequences enhances retroviral vector titer and expression. Schambach A, Bohne J, Baum C, Hermann FG, Egerer L, von Laer D, Giroglou T. Gene Ther. 2006 Apr;13(7):641-5.'

Addgene Sanger sequencing confirms that all four ATGs in the oPRE have been mutated to TAGs