secretedBirA-8his
(Plasmid #32408)

• Depositing Lab: Gavin Wright
• Publication: Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BirA
• Alt name: Biotin ligase
• Species: E. coli
• Insert Size (bp): 1121
• Tag / Fusion Protein:
  • 8 His tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (destroyed during cloning)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry