CD200Cd4d3+4-bio
(Plasmid #32404)

• Purpose: A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit
• Depositing Lab: Gavin Wright
• Publication: Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.

Backbone

• Vector backbone: pEMCS
• Backbone manufacturer: Mark Bushell
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CD200
• Alt name: OX-2
• Species: R. norvegicus (rat)
• Insert Size (bp): 714
• Promoter: CMV
• Tags / Fusion Proteins:
  • Cd4d3+4 (C terminal on insert)
  • biotinylation peptide (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing result with the LNCX primer found a single nucleotide mismatch at bp# 6587 when compared to the full plasmid sequence provided by the depositing scientist. According to the depositing scientist, the mismatch is in the Kozak sequence preceding the CD200bio open reading frame and should not affect protein expression.