CD4d3+4-blac
(Plasmid #32403)

• Purpose: (Empty Backbone)
• Depositing Lab: Gavin Wright
• Publication: Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.

Backbone

• Vector backbone: pEMCS
• Backbone manufacturer: Mark Bushell
• Backbone size (bp): 6000
• Vector type: Mammalian Expression
• Promoter: CMV promoter
• Tags / Fusion Proteins:
  • ratCd4d3+4 (C terminal on insert)
  • pentamerization domain of rat cartilaginous oligomeric matrix protein (COMP) (C terminal on insert)
  • beta-lactamase (C terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing result with the rCD4-F primer found a single nucleotide mismatch at bp# 858, when compared to the full plasmid sequence provided by the depositing scientist. According to the depositing scientist, this is a silent mutation in the COMP domain of the protein and should not affect protein expression.