CD4d3+4-bio
(Plasmid #32402)

• Purpose: (Empty Backbone)
• Depositing Lab: Gavin Wright
• Publication: Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.

Backbone

• Vector backbone: pEMCS
• Backbone manufacturer: Mark Bushell
• Backbone size (bp): 6000
• Vector type: Mammalian Expression
• Promoter: CMV promoter
• Tags / Fusion Proteins:
  • ratCd4d3+4 (C terminal on insert)
  • biotinylation peptide (C terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing result found a 60 nucleotide insert between bp#750/751 when compared to the full plasmid sequence provided by the depositing scientist. The depositing scientist confirmed that Addgene's sequence is correct--the insertion is located downstream of the CD4 bio coding sequence and is not a concern for protein expression.