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Addgene

CX-nestinGFPas
(Plasmid #32401)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32401 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    CX lentiviral vector
  • Modifications to backbone
    insertion of the whole nestin promoter-GFP-nestin enhancer and betaglobin polyA into a CX lentiviral vector
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nestin promoter/enhancer GFP
  • Insert Size (bp)
    5900

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer gacagagacagatccattc
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that there are some discrepancies between Addgene's QC sequence and the depositor's assembled sequence. The plasmid should still function as reported.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CX-nestinGFPas was a gift from Patrick Salmon & Henryk Zulewski (Addgene plasmid # 32401 ; http://n2t.net/addgene:32401 ; RRID:Addgene_32401)
  • For your References section:

    Multipotential nestin and Isl-1 positive mesenchymal stem cells isolated from human pancreatic islets. Eberhardt M, Salmon P, von Mach MA, Hengstler JG, Brulport M, Linscheid P, Seboek D, Oberholzer J, Barbero A, Martin I, Muller B, Trono D, Zulewski H. Biochem Biophys Res Commun. 2006 Jul 7;345(3):1167-76. Epub 2006 May 11. 10.1016/j.bbrc.2006.05.016 PubMed 16713999