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pGL3-p107 WT
(Plasmid #32392)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32392 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p107 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    900
  • Entrez Gene
    Rbl1 (a.k.a. PRB1, p107)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer Luc_N_Rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-p107 WT was a gift from Julien Sage (Addgene plasmid # 32392 ; http://n2t.net/addgene:32392 ; RRID:Addgene_32392)

  • For your References section:

    Regulation of RB transcription in vivo by RB family members. Burkhart DL, Ngai LK, Roake CM, Viatour P, Thangavel C, Ho VM, Knudsen ES, Sage J. Mol Cell Biol. 2010 Apr;30(7):1729-45. Epub 2010 Jan 25. 10.1128/MCB.00952-09 PubMed 20100864
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