Skip to main content
Addgene

pGL3-Rb WT
(Plasmid #32390)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32390 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rb promoter
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Rb1 (a.k.a. Rb, Rb-1, p110-RB1, pRb, pp105)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer Luc_N_Rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-Rb WT was a gift from Julien Sage (Addgene plasmid # 32390 ; http://n2t.net/addgene:32390 ; RRID:Addgene_32390)

  • For your References section:

    Regulation of RB transcription in vivo by RB family members. Burkhart DL, Ngai LK, Roake CM, Viatour P, Thangavel C, Ho VM, Knudsen ES, Sage J. Mol Cell Biol. 2010 Apr;30(7):1729-45. Epub 2010 Jan 25. 10.1128/MCB.00952-09 PubMed 20100864
Related items:
Commonly requested with: