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pGL3-p107 1*2*
(Plasmid #32387)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32387 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p107 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    900
  • Mutation
    1st and 2nd E2F binding sites mutated: GCG-->AAA and GTC-->AAA
  • Entrez Gene
    Rbl1 (a.k.a. PRB1, p107)
  • Tag / Fusion Protein
    • Luciferase (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer Luc_N_Rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-p107 1*2* was a gift from Julien Sage (Addgene plasmid # 32387 ; http://n2t.net/addgene:32387 ; RRID:Addgene_32387)

  • For your References section:

    Tandem E2F binding sites in the promoter of the p107 cell cycle regulator control p107 expression and its cellular functions. Burkhart DL, Wirt SE, Zmoos AF, Kareta MS, Sage J. PLoS Genet. 2010 Jun 24;6(6):e1001003. 10.1371/journal.pgen.1001003 PubMed 20585628
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