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Addgene

pML1357
(Plasmid #32378)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32378 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pML603
  • Vector type
    bacterial integration vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    int Giles; gfpm2+; xylEm; ttsbiA, ttsbiB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MLU I (not destroyed)
  • 3′ cloning site ASC I (not destroyed)
  • 5′ sequencing primer Lox Pfw
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is an improved Giles integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE as reporter genes; terminators to protect expression cassette against transcriptional
interference.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pML1357 was a gift from Michael Niederweis (Addgene plasmid # 32378 ; http://n2t.net/addgene:32378 ; RRID:Addgene_32378)

  • For your References section:

    Taking phage integration to the next level as a genetic tool for mycobacteria. Huff J, Czyz A, Landick R, Niederweis M. Gene. 2010 Nov 15;468(1-2):8-19. Epub 2010 Aug 6. 10.1016/j.gene.2010.07.012 PubMed 20692326
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