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pCDNA3 5' cMyc- SMCI-S966A
(Plasmid #32371)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3 cMyc
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMC1A
  • Alt name
    SMCB
  • Alt name
    CDLS2
  • Alt name
    SMCILI
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3700
  • Mutation
    S996A
  • GenBank ID
    8243
  • Entrez Gene
    SMC1A (a.k.a. CDLS2, DEE85, DXS423E, EIEE85, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB)
  • Tag / Fusion Protein
    • c-Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ECORl (not destroyed)
  • 3′ cloning site Xhol (not destroyed)
  • 5′ sequencing primer CMV-f
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 5' cMyc- SMCI-S966A was a gift from Michael Kastan (Addgene plasmid # 32371 ; http://n2t.net/addgene:32371 ; RRID:Addgene_32371)

  • For your References section:

    Involvement of the cohesin protein, Smc1, in Atm-dependent and independent responses to DNA damage. Kim ST, Xu B, Kastan MB. Genes Dev. 2002 Mar 1;16(5):560-70. 10.1101/gad.970602 PubMed 11877376
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