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Addgene

pMN437
(Plasmid #32362)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32362 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMS2
  • Vector type
    Mycobacterial GFP expression vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gfpm2+
  • Alt name
    codon optimized GFP for mycobacteria

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is an expression vector with a codon optimized gfp gene encoding an improved GFP (enhanced fluorescence, faster folding, more stable).

Additional reference for this construct: Steinhauer et al., 2010; Appl Environ Microbiol 76:546-554

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMN437 was a gift from Michael Niederweis (Addgene plasmid # 32362 ; http://n2t.net/addgene:32362 ; RRID:Addgene_32362)

  • For your References section:

    Identification of outer membrane proteins of Mycobacterium tuberculosis. Song H, Sandie R, Wang Y, Andrade-Navarro MA, Niederweis M. Tuberculosis (Edinb). 2008 Nov;88(6):526-44. Epub 2008 Apr 25. 10.1016/j.tube.2008.02.004 PubMed 18439872
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