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Addgene

pMycVec1
(Plasmid #32360)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32360 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMS2Kan
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ColE1 origin replication

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Ava I (not destroyed)
  • 3′ cloning site Dra I (not destroyed)
  • 5′ sequencing primer ColE1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a mycobacterial shuttle vector with a lower copy number in E. coli.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMycVec1 was a gift from Michael Niederweis (Addgene plasmid # 32360 ; http://n2t.net/addgene:32360 ; RRID:Addgene_32360)

  • For your References section:

    Energy transfer between fluorescent proteins using a co-expression system in Mycobacterium smegmatis. Kaps I, Ehrt S, Seeber S, Schnappinger D, Martin C, Riley LW, Niederweis M. Gene. 2001 Oct 31;278(1-2):115-24. 10.1016/S0378-1119(01)00712-0 PubMed 11707328
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