pBH-UAS-secA5-YFP
(Plasmid #32359)

• Purpose: Live imaging of apoptotic cells in zebrafish
• Depositing Lab: Randall Peterson
• Publication: van Ham et al FASEB J. 2010 Nov;24(11):4336-42. Epub 2010 Jul 2.

Backbone

• Vector backbone: pBH-UAS-gtwy-YFP
• Backbone manufacturer: Michael L. Nonet
• Vector type: Zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: secreted human annexin 5
• Species: H. sapiens (human)
• Entrez Gene: ANXA5 (a.k.a. ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph)
• Promoter: UAS

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: cctcgcccttgctcaccat

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The backbone plasmid (pBH-UAS-gtwy-YFP) was created by the Michael L. Nonet lab (Wustl).
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

http://neuroscience.wustl.edu/nonetlab/ResourcesF/Zfseqinfo.html