Skip to main content
Addgene

pDESTsvaw
(Plasmid #32318)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32318 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pJFRC7

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ccdB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xba I (destroyed during cloning)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see http://www.everyvector.com/sequences/show_public/13276 for additional plasmid map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDESTsvaw was a gift from Steve Stowers (Addgene plasmid # 32318 ; http://n2t.net/addgene:32318 ; RRID:Addgene_32318)

  • For your References section:

    A Gateway MultiSite Recombination Cloning Toolkit. Petersen LK, Stowers RS. PLoS One. 2011;6(9):e24531. Epub 2011 Sep 9. 10.1371/journal.pone.0024531 PubMed 21931740
Related items:
Commonly requested with: