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Addgene

pDESThaw
(Plasmid #32317)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32317 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSC5

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ccdB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NsiI (destroyed during cloning)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see http://www.everyvector.com/sequences/show_public/12784 for additional plasmid map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDESThaw was a gift from Steve Stowers (Addgene plasmid # 32317 ; http://n2t.net/addgene:32317 ; RRID:Addgene_32317)

  • For your References section:

    A Gateway MultiSite Recombination Cloning Toolkit. Petersen LK, Stowers RS. PLoS One. 2011;6(9):e24531. Epub 2011 Sep 9. 10.1371/journal.pone.0024531 PubMed 21931740
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