pENTR L3-4XmCherry-HA-L2
(Plasmid
#32315)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32315 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR 221 P3-P2
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2557
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Vector typeGateway MultiSite entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name4XmCherry-HA
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Insert Size (bp)2910
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see http://www.everyvector.com/sequences/show_public/12938 for additional plasmid map.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR L3-4XmCherry-HA-L2 was a gift from Steve Stowers (Addgene plasmid # 32315 ; http://n2t.net/addgene:32315 ; RRID:Addgene_32315) -
For your References section:
A Gateway MultiSite Recombination Cloning Toolkit. Petersen LK, Stowers RS. PLoS One. 2011;6(9):e24531. Epub 2011 Sep 9. 10.1371/journal.pone.0024531 PubMed 21931740
