pENTR R4-QF-R3
(Plasmid
#32310)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32310 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR 221 P4r-P3r
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2667
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Vector typeGateway MultiSite entry clone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameQF
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SpeciesNeurospora crassa
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Insert Size (bp)2455
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see http://www.everyvector.com/sequences/show_public/12866 for additional plasmid map.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR R4-QF-R3 was a gift from Steve Stowers (Addgene plasmid # 32310 ; http://n2t.net/addgene:32310 ; RRID:Addgene_32310) -
For your References section:
A Gateway MultiSite Recombination Cloning Toolkit. Petersen LK, Stowers RS. PLoS One. 2011;6(9):e24531. Epub 2011 Sep 9. 10.1371/journal.pone.0024531 PubMed 21931740
