hATMS1981A
(Plasmid #32300)

• Depositing Lab: Michael Kastan
• Publication: Bakkenist et al Nature. 2003 Jan 30;421(6922):499-506.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Growth instructions: see attachment
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ATM
• Alt name: ATI
• Alt name: ATA
• Alt name: ATDC
• Species: H. sapiens (human)
• Insert Size (bp): 9581
• Mutation: S1981A
• Entrez Gene: ATM (a.k.a. AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1)
• Tags / Fusion Proteins:
  • Flag (N terminal on backbone)
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI-SAl I (destroyed during cloning)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHrev

Resource Information

• Supplemental Documents:
  • Transformation of ATM Plasmid
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry