pMS26
(Plasmid #32295)

• Purpose: (Empty Backbone)
• Depositing Lab: Elisabeth Raleigh
• Publication: Sibley et al Nucleic Acids Res. 2012 Feb;40(3):e19. doi: 10.1093/nar/gkr1085. Epub 2011 Nov 28.

Backbone

• Vector backbone: pGRG36
• Backbone manufacturer: none
• Backbone size (bp): 12459
• Modifications to backbone: addition of translation and transcription terminators, multicloning site including USERBstBI rapid cloning strategy sites, regulatable promoter rhaBp.
• Vector type: Bacterial Expression ; bacterial gene addition
• Promoter: rhaB

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Parent plasmid pGRG36 was obtained from Nancy Craig Dept. of Molecular Biology and Genetics Howard Hughes Medical Institute Johns Hopkins School of Medicine Baltimore, MD 21205-2185

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Insulation and cloning region designed by Sibley and Raleigh.

The strain containing the plasmid is actually

F’proAB lacIq Δ(lacZ)M15 zzf::miniTn10 (KanR)/ fhuA2 Δ(lacI-lacA)200 glnV44 e14- rfbD1? relA1? endA1 spoT1? thi-1 ∆(mcrC-mrr)114::IS10

Lin, L.F., Posfai, J., Roberts, R.J. and Kong, H. (2001) Comparative genomics of the restriction-modification systems in Helicobacter pylori. Proc Natl Acad Sci U S A, 98, 2740-2745.