pMR150
(Plasmid
#32292)
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32292 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript II KS
- Backbone size w/o insert (bp) 2961
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegenomic methylation probe-minor satellite repeat
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SpeciesM. musculus (mouse)
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Insert Size (bp)150
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (unknown if destroyed)
- 3′ cloning site PstI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Induction of tumors in mice by genomic hypomethylation. Gaudet F, Hodgson JG, Eden A, Jackson-Grusby L, Dausman J, Gray JW, Leonhardt H, Jaenisch R. Science. 2003 Apr 18;300(5618):489-92.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMR150 was a gift from Verne Chapman & Rudolf Jaenisch (Addgene plasmid # 32292 ; http://n2t.net/addgene:32292 ; RRID:Addgene_32292) -
For your References section:
Differences in DNA methylation during oogenesis and spermatogenesis and their persistence during early embryogenesis in the mouse. Sanford JP, Clark HJ, Chapman VM, Rossant J. Genes Dev. 1987 Dec;1(10):1039-46. 10.1101/gad.1.10.1039 PubMed 3428592