TALEN1258
(Plasmid
#32281)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32281 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneJDS78
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Vector typeMammalian Expression ; T7
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTAL array targeting gria3a
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Alt namegria3a_L (TAL 1258)
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Alt namegria3a
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SpeciesD. rerio (zebrafish)
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Entrez Genegria3a (a.k.a. CH73-166N24.1, gria3.1, zfGRIA3[a])
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Tags
/ Fusion Proteins
- 3X Flag (N terminal on backbone)
- FOKI WT (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer CMV-f
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
FOKI: WT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TALEN1258 was a gift from Keith Joung (Addgene plasmid # 32281 ; http://n2t.net/addgene:32281 ; RRID:Addgene_32281) -
For your References section:
Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR. Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. 10.1038/nbt.1934 PubMed 21822241