TALEN1257
(Plasmid #32280)

• Depositing Lab: Keith Joung
• Publication: Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.

Backbone

• Vector backbone: JDS78
• Vector type: Mammalian Expression ; T7

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TAL array targeting hey2
• Alt name: hey2_R (TAL 1257)
• Alt name: hey2
• Species: D. rerio (zebrafish)
• Entrez Gene: hey2 (a.k.a. grl, zgc:136746)
• Tags / Fusion Proteins:
  • 3X FLAG (N terminal on backbone)
  • WT FOKI (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: CMV-f
• 3′ sequencing primer: BGH rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
FOKI: WT