TAL016
(Plasmid
#32259)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32259 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57-ΔBsaI
- Backbone size w/o insert (bp) 2708
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Modifications to backboneSilent valine mutation (C->G) at position 1794 to destroy alternate BsaI restriction site in pUC57
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTAL016
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Alt nameI (Type III - A/NI)
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Alt nameγ / SNI / A
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Insert Size (bp)119
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (destroyed during cloning)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer OK163 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TAL016 was a gift from Keith Joung (Addgene plasmid # 32259 ; http://n2t.net/addgene:32259 ; RRID:Addgene_32259) -
For your References section:
Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR. Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. 10.1038/nbt.1934 PubMed 21822241