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Addgene

pMCGL-T7Mutant
(Plasmid #32245)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM4Z
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Glucosamine-6-phosphate activated ribozyme
  • Alt name
    glmS Ribozyme
  • Species
    Bacillus subtilis
  • Insert Size (bp)
    211

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AflII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCGL-T7Mutant was a gift from Nils Walter (Addgene plasmid # 32245 ; http://n2t.net/addgene:32245 ; RRID:Addgene_32245)

  • For your References section:

    Nondenaturing purification of co-transcriptionally folded RNA avoids common folding heterogeneity. Pereira MJ, Behera V, Walter NG. PLoS One. 2010 . 5(9):e12953. 10.1371/journal.pone.0012953 PubMed 20886091
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