1099-N-cadherin-AAA-YFP
(Plasmid #32237)

• Depositing Lab: Kathleen Green
• Publication: Chen et al J Cell Biol. 2003 Nov 10;163(3):547-57.

Backbone

• Vector backbone: pEYFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: N-cadherin
• Species: M. musculus (mouse)
• Mutation: Glu–Glu–Asp (aa 780–782) ---> Ala–Ala–Ala
• Entrez Gene: Cdh2 (a.k.a. CDHN, N-CAD, Ncad)
• Promoter: CMV
• Tag / Fusion Protein:
  • EYFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: EXFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry