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Addgene

pML597
(Plasmid #32235)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32235 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMN234
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mflpe
  • Mutation
    M.tuberculosis codon optimized flpe gene

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer imyc_seq
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This contains a codon optimized flpe gene encoding an enhanced Flp recombinase for efficient excision of FRT-flanked inserts in mycobacteria.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pML597 was a gift from Michael Niederweis (Addgene plasmid # 32235 ; http://n2t.net/addgene:32235 ; RRID:Addgene_32235)

  • For your References section:

    Functional expression of the Flp recombinase in Mycobacterium bovis BCG. Song H, Niederweis M. Gene. 2007 Sep 15;399(2):112-9. Epub 2007 May 21. 10.1016/j.gene.2007.05.005 PubMed 17590537
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