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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32232 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneb-actin vector
- Backbone size w/o insert (bp) 9500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDesmoglein2
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Alt nameDSG2
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SpeciesH. sapiens (human)
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Insert Size (bp)3362
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Entrez GeneDSG2 (a.k.a. CDHF5, HDGC)
- Promoter human beta-actin
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Tag
/ Fusion Protein
- Myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer hBactin-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
726-Desmoglein2-myc was a gift from Kathleen Green (Addgene plasmid # 32232 ; http://n2t.net/addgene:32232 ; RRID:Addgene_32232) -
For your References section:
Assembly of desmosomal cadherins into desmosomes is isoform dependent. Ishii K, Norvell SM, Bannon LJ, Amargo EV, Pascoe LT, Green KJ. J Invest Dermatol. 2001 Jul;117(1):26-35. 10.1046/j.0022-202x.2001.01400.x PubMed 11442746
Map uploaded by the depositor.
