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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32228 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLK 444
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePlakoglobin
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SpeciesH. sapiens (human)
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Insert Size (bp)2235
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Entrez GeneJUP (a.k.a. CTNNG, DP3, DPIII, PDGB, PG, PKGB)
- Promoter human beta-actin
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Tag
/ Fusion Protein
- Myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer hBactin-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
330-Plakoglobin-Myc was a gift from Kathleen Green (Addgene plasmid # 32228 ; http://n2t.net/addgene:32228 ; RRID:Addgene_32228) -
For your References section:
Roles of plakoglobin end domains in desmosome assembly. Palka HL, Green KJ. J Cell Sci. 1997 Oct;110 ( Pt 19):2359-71. PubMed 9410875