1136- Desmoplakin-GFP
(Plasmid #32227)

• Depositing Lab: Kathleen Green
• Publication: Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Desmoplakin
• Species: H. sapiens (human)
• Insert Size (bp): 8616
• Entrez Gene: DSP (a.k.a. DCWHKTA, DP)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry