pAd GFP HDAC5 S259A/S498A
(Plasmid #32218)

• Depositing Lab: Reuben Shaw
• Publication: Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Backbone

• Vector backbone: pAd CMV/V5 DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 37000
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HDAC5
• Species: H. sapiens (human)
• Insert Size (bp): 3400
• Mutation: S259A/S498A
• GenBank ID: NM_001015053
• Entrez Gene: HDAC5 (a.k.a. HD5, NY-CO-9)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: GFP-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Subcloned and converted into a Gateway plasmid from original plasmid described previously in Berdeux et al. PMID 17468767
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry