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Addgene

pTALEN_v2 (NI)
(Plasmid #32189)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32189 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TALE Nuclease, NI for 0.5 repeat

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTALEN_v2 (NI) was a gift from Feng Zhang (Addgene plasmid # 32189 ; http://n2t.net/addgene:32189 ; RRID:Addgene_32189)
  • For your References section:

    A transcription activator-like effector toolbox for genome engineering. Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. 10.1038/nprot.2011.431 PubMed 22222791