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LHP2691 - pET30-Ub-K63A
(Plasmid #32179)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32179 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-30 Xa/LIC
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5450
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synthetic yeast Ubiquitin
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    K63A
  • Entrez Gene
    UBA1 (a.k.a. YKL210W)
  • Entrez Gene
    UBA2 (a.k.a. YDR390C, UAL1)
  • Promoter T7
  • Tag / Fusion Protein
    • HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LHP2691 - pET30-Ub-K63A was a gift from Linda Hicke (Addgene plasmid # 32179 ; http://n2t.net/addgene:32179 ; RRID:Addgene_32179)
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