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Addgene

pMN252
(Plasmid #32177)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32177 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMN250
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rpsL, FRT-hyg-FRT

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sph I (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer ColE2
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a suicide vector for gene deletions in mycobacteria; requires rpsL mutant (streptomycin resistant)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMN252 was a gift from Michael Niederweis (Addgene plasmid # 32177 ; http://n2t.net/addgene:32177 ; RRID:Addgene_32177)

  • For your References section:

    Consecutive gene deletions in Mycobacterium smegmatis using the yeast FLP recombinase. Stephan J, Stemmer V, Niederweis M. Gene. 2004 Dec 8;343(1):181-90. 10.1016/j.gene.2004.08.028 PubMed 15563844
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