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Addgene

LHP905 - Gal1-Ub
(Plasmid #32173)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32173 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synthetic yeast Ubiquitin
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    UBA1 (a.k.a. YKL210W)
  • Entrez Gene
    UBA2 (a.k.a. YDR390C, UAL1)
  • Promoter Gal1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Gal1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CEN4 ARS1 S. cerevisiae replication.

Ubiquitin was amplified using oligos which introduce BamHI sites on either end and cloned in to the BamHI site on the vector. Insertion was confirmed by digests and then orientation confirmed by sequencing.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LHP905 - Gal1-Ub was a gift from Linda Hicke (Addgene plasmid # 32173 ; http://n2t.net/addgene:32173 ; RRID:Addgene_32173)
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