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LHP428-YCplac33-STE2-378Stop
(Plasmid #32172)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32172 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YCPlac33
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STE2
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Stop codon introduced at aa 378
  • Entrez Gene
    STE2 (a.k.a. YFL026W)
  • Promoter STE2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer M13_puc_Fwd
  • 3′ sequencing primer M13_puc_Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

STE2 ORF with a stop codon introduced at aa378. The 1.6kB STE2 378Stop -encoding HindIII fragment was subcloned from LHP147 into the HindIII site of YCplac33. The upstream promoter sequence was obtained by dropping out the 1260bp EcoRI(upstream)/PstI fragment and ligating in the 2KB EcoRI/PstI fragment from pJR3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LHP428-YCplac33-STE2-378Stop was a gift from Linda Hicke (Addgene plasmid # 32172 ; http://n2t.net/addgene:32172 ; RRID:Addgene_32172)
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