LHP424-YCplac33-STE2-WT
(Plasmid
#32171)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32171 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneYCPlac33
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSTE2
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneSTE2 (a.k.a. YFL026W)
- Promoter STE2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer M13_puc_Fwd
- 3′ sequencing primer M13_puc_Rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Wildtype STE2 ORF including 1100bp upstream of the start codon (from SalI site) and 200bp downstream of the stop codon (HindIII). The wildtype 1.6kB HindIII fragment encoding STE2 was subcloned from LHP123 into the HindIII site of YCplac33. The upstream promoter sequence was obtained by dropping out the 1260bp EcoRI (upstream)/PstI fragment and ligating in the 2kB EcoRI/PstI fragment from pJR3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LHP424-YCplac33-STE2-WT was a gift from Linda Hicke (Addgene plasmid # 32171 ; http://n2t.net/addgene:32171 ; RRID:Addgene_32171)