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Addgene

LHP599 - pACT2-Ub
(Plasmid #32170)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32170 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACT2 AD
  • Backbone manufacturer
    Clontech - Discontinued
  • Backbone size w/o insert (bp) 8100
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synthetic yeast Ubiquitin
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    UBA1 (a.k.a. YKL210W)
  • Entrez Gene
    UBA2 (a.k.a. YDR390C, UAL1)
  • Promoter pADH1
  • Tags / Fusion Proteins
    • Gal4 AD (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Gal4_AD_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LHP599 - pACT2-Ub was a gift from Linda Hicke (Addgene plasmid # 32170 ; http://n2t.net/addgene:32170 ; RRID:Addgene_32170)
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