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Addgene

LHP416-PAN1-HA
(Plasmid #32159)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32159 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PAN1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    PAN1 (a.k.a. YIR006C, DIM2, MDP3, MIP3)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site StuI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer HA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PAN1-HA including 1kB upstream sequence and 280bp downstream sequence of PAN1 ORF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LHP416-PAN1-HA was a gift from Linda Hicke (Addgene plasmid # 32159 ; http://n2t.net/addgene:32159 ; RRID:Addgene_32159)
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