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LHP308 - Ub - wt
(Plasmid #32151)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32151 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pES7
  • Backbone manufacturer
    Mike Ellison - University of Alberta
  • Modifications to backbone
    The c-myc epitope tag was removed
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synthetic yeast Ubiquitin
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    UBA1 (a.k.a. YKL210W)
  • Entrez Gene
    UBA2 (a.k.a. YDR390C, UAL1)
  • Promoter CUP1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CUP1 forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BamHI-KpnI fragment from YEp96 (D. Ecker, U PENN) was cloned into pES7 (Mike Ellison, University of Alberta) cut with BamHI/KpnI.

2 µm S. cerevisiae origin

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LHP308 - Ub - wt was a gift from Linda Hicke (Addgene plasmid # 32151 ; http://n2t.net/addgene:32151 ; RRID:Addgene_32151)

  • For your References section:

    A function for monoubiquitination in the internalization of a G protein-coupled receptor. Terrell J, Shih S, Dunn R, Hicke L. Mol Cell. 1998 Jan;1(2):193-202. 10.1016/S1097-2765(00)80020-9 PubMed 9659916
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