pJW1
(Plasmid
#32130)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 32130 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJW1
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Tag
/ Fusion Protein
- FLAG
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BW25142 (a lambda pir strain)
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Growth instructionsFor information on growth strain: (http://cgsc.biology.yale.edu/Strain.php?ID=70715)
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJW1 was a gift from Eric Rubin (Addgene plasmid # 32130 ; http://n2t.net/addgene:32130 ; RRID:Addgene_32130) -
For your References section:
Depletion of antibiotic targets has widely varying effects on growth. Wei JR, Krishnamoorthy V, Murphy K, Kim JH, Schnappinger D, Alber T, Sassetti CM, Rhee KY, Rubin EJ. Proc Natl Acad Sci U S A. 2011 Mar 8;108(10):4176-81. Epub 2011 Feb 22. 10.1073/pnas.1018301108 PubMed 21368134