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Addgene

413-Met25 Kar2 TorsinA K108A HDEL
(Plasmid #32121)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32121 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    413-Met25
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TorsinA
  • Species
    H. sapiens (human)
  • Mutation
    K108A
  • Entrez Gene
    TOR1A (a.k.a. AMC5, DQ2, DYT1)
  • Tags / Fusion Proteins
    • Kar2 (N terminal on insert)
    • HDEL (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer m13-r
  • 3′ sequencing primer m13-f
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    413-Met25 Kar2 TorsinA K108A HDEL was a gift from Susan Lindquist (Addgene plasmid # 32121 ; http://n2t.net/addgene:32121 ; RRID:Addgene_32121)

  • For your References section:

    TorsinA and the TorsinA-Interacting Protein Printor Have No Impact on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast. Valastyan JS, Lindquist S. PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26. 10.1371/journal.pone.0022744 PubMed 21818381
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