ph3b
(Plasmid
#32110)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32110 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonephGf
- Backbone size w/o insert (bp) 4508
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBKV VP3
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Alt nameBKPyV
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Alt nameBK Polyomavirus
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Speciesvirus
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Insert Size (bp)698
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Mutationcodon modified
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please consult laboratory website, http://home.ccr.cancer.gov/Lco/plasmids.asp, for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ph3b was a gift from Christopher Buck (Addgene plasmid # 32110 ; http://n2t.net/addgene:32110 ; RRID:Addgene_32110) -
For your References section:
Glycosaminoglycans and sialylated glycans sequentially facilitate merkel cell polyomavirus infectious entry. Schowalter RM, Pastrana DV, Buck CB. PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28. 10.1371/journal.ppat.1002161 PubMed 21829355