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Addgene

pMN234
(Plasmid #32108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32108 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMN210
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    flpe
  • Promoter imyc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pme (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer pMS-seq1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is an expression vector for an enhanced Flp recombinase to excise FRT-flanked inserts in mycobacteria.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMN234 was a gift from Michael Niederweis (Addgene plasmid # 32108 ; http://n2t.net/addgene:32108 ; RRID:Addgene_32108)

  • For your References section:

    Consecutive gene deletions in Mycobacterium smegmatis using the yeast FLP recombinase. Stephan J, Stemmer V, Niederweis M. Gene. 2004 Dec 8;343(1):181-90. 10.1016/j.gene.2004.08.028 PubMed 15563844
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