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Addgene

CbS-Flag-TRRAP
(Plasmid #32103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32103 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CbS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Better growth in JM109 (Primeplus cells)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Transformation/transcription domain-associated protein
  • Alt name
    TRRAP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    16828
  • Entrez Gene
    TRRAP (a.k.a. DEDDFA, DFNA75, PAF350/400, PAF400, STAF40, TR-AP, Tra1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag 1x (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site not1 (not destroyed)
  • 3′ cloning site cla1 (not destroyed)
  • 5′ sequencing primer CAG ATC GCC TGG AGA CGC C
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Constructed by Michael D. Cole Lab.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CbS-Flag-TRRAP was a gift from Michael Cole & Yardena Samuels (Addgene plasmid # 32103 ; http://n2t.net/addgene:32103 ; RRID:Addgene_32103)
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