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Addgene

pMslc
(Plasmid #32095)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32095 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMONO-blasti
  • Backbone manufacturer
    InvivoGen
  • Backbone size w/o insert (bp) 3435
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Blasticidin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLC35A1
  • Alt name
    solute carrier family 35 (CMP-sialic acid transporter), member A1
  • Alt name
    CMP-sialic acid transporter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1839
  • Entrez Gene
    SLC35A1 (a.k.a. CDG2F, CMPST, CST, hCST)
  • Promoter FerH

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer TGAGCGGAGCTAATTCTCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please consult laboratory website, http://home.ccr.cancer.gov/Lco/plasmids.asp, for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMslc was a gift from Christopher Buck (Addgene plasmid # 32095 ; http://n2t.net/addgene:32095 ; RRID:Addgene_32095)

  • For your References section:

    Glycosaminoglycans and sialylated glycans sequentially facilitate merkel cell polyomavirus infectious entry. Schowalter RM, Pastrana DV, Buck CB. PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28. 10.1371/journal.ppat.1002161 PubMed 21829355
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