pwB2b
(Plasmid #32094)

• Depositing Lab: Christopher Buck
• Publication: Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28.

Backbone

• Vector backbone: pGwf
• Backbone size w/o insert (bp): 5338
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: BKV VP1
• Alt name: BK polyomavirus
• Alt name: BKPyV
• Species: virus
• Insert Size (bp): 1088
• Mutation: codon modified

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: attR1
• 3′ sequencing primer: attR2

Gene/Insert 2

• Gene/Insert name: BKV VP2
• Species: virus
• Insert Size (bp): 1055
• Mutation: codon modified

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please consult laboratory website, http://home.ccr.cancer.gov/Lco/plasmids.asp, for more information.