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Addgene

pWZLFlagMenin(S394A, T397A, S399A)
(Plasmid #32086)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32086 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWZL
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Menin
  • Mutation
    S394A, T397A, S399A
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZLFlagMenin(S394A, T397A, S399A) was a gift from Matthew Meyerson (Addgene plasmid # 32086 ; http://n2t.net/addgene:32086 ; RRID:Addgene_32086)

  • For your References section:

    The menin tumor suppressor protein is phosphorylated in response to DNA damage. Francis J, Lin W, Rozenblatt-Rosen O, Meyerson M. PLoS One. 2011 . 6(1):e16119. 10.1371/journal.pone.0016119 PubMed 21264250
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