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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMDC32
- Backbone size (bp) 12096
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Modifications to backboneHPB tag was added to allow its fusion to gene of interest for expression in plant.
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Vector typeGateway destination binary vector
- Promoter 35S promoter
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Selectable markersHygromycin
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Tag
/ Fusion Protein
- HA-Prescission-Biotin (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberHigh Copy
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMDC32-HPB was a gift from Fumi Katagiri (Addgene plasmid # 32078 ; http://n2t.net/addgene:32078 ; RRID:Addgene_32078) -
For your References section:
Purification of low-abundance Arabidopsis plasma-membrane protein complexes and identification of candidate components. Qi Y, Katagiri F. Plant J. 2009 Mar;57(5):932-44. Epub 2008 Dec 4. 10.1111/j.1365-313X.2008.03736.x PubMed 19000159
Map uploaded by the depositor.
