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Addgene

pBabe EGFR Del4(T790M)
(Plasmid #32074)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32074 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABEpuro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3600
  • Mutation
    Delete L747-P753, insert S, T790M
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaB1 (destroyed during cloning)
  • 3′ cloning site SnaB1 (destroyed during cloning)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe EGFR Del4(T790M) was a gift from Matthew Meyerson (Addgene plasmid # 32074 ; http://n2t.net/addgene:32074 ; RRID:Addgene_32074)

  • For your References section:

    Allele-dependent variation in the relative cellular potency of distinct EGFR inhibitors. Yuza Y, Glatt KA, Jiang J, Greulich H, Minami Y, Woo MS, Shimamura T, Shapiro G, Lee JC, Ji H, Feng W, Chen TH, Yanagisawa H, Wong KK, Meyerson M. Cancer Biol Ther. 2007 May . 6(5):661-7. 10.4161/cbt.6.5.4003 PubMed 17495523
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